Journal Article

A rapid method of impregnating endotracheal tubes and urinary catheters with gendine: a novel antiseptic agent

Gassan Chaiban, Hend Hanna, Tanya Dvorak and Issam Raad

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 1, pages 51-56
Published in print January 2005 | ISSN: 0305-7453
Published online January 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh499
A rapid method of impregnating endotracheal tubes and urinary catheters with gendine: a novel antiseptic agent

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  • Medical Oncology
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Objectives: To test the efficacy of gendine, a novel antiseptic, containing Gentian Violet and chlorhexidine, in coating different medical devices, including endotracheal tubes (ETT) and urinary catheters (UC).

Methods: We determined the antimicrobial efficacy and cytotoxicity of ETT and UC segments coated, through an instant dip method, with gendine. Using the modified Kirby–Bauer method, gendine-coated devices showed zones of inhibition of ≥15 mm against methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Escherichia coli and Candida parapsilosis.

Results: Gendine-coated endotracheal tubes (GND-ETT) soaked in bronchoalveolar fluid (BAL) and incubated at 37°C maintained a zone of inhibition of ≥15 mm against MRSA and P. aeruginosa for at least 3 weeks. Similarly, gendine-coated urinary catheters (GND-UC), soaked in urine, maintained a zone of inhibition of ≥15 mm against E. coli for 8 weeks. Using the minimum essential media elution method in mouse fibroblast cells, GND-ETT and GND-UC were found to be non-cytotoxic. Gendine-coated UC significantly reduced the amount of viable MRSA, E. coli or C. parapsilosis organisms adhering to their surfaces when compared with silver/hydrogel-coated urinary catheters or control uncoated catheters (P < 0.01). Similarly GND-ETT significantly reduced the adherence of the same organisms as well as P. aeruginosa when compared with control (P ≤ 0.02).

Conclusions: GND-ETT and GND-UC impregnated using an instantaneous dip method, were shown to have broad-spectrum activity, prolonged antimicrobial durability and high efficacy in inhibiting adherence of organisms commonly causing nosocomial pneumonia and urinary tract infection. Furthermore, these coated devices were shown to be non-cytotoxic.

Keywords: Gentian Violet; medical devices; novel antiseptics; antimicrobial activity; bacterial adherence

Journal Article.  4094 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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