Journal Article

A nosocomial outbreak of <i>Acinetobacter baumannii</i> isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58

Claire Héritier, Anne Dubouix, Laurent Poirel, Nicole Marty and Patrice Nordmann

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 1, pages 115-118
Published in print January 2005 | ISSN: 0305-7453
Published online January 2005 | e-ISSN: 1460-2091 | DOI:
A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58

More Like This

Show all results sharing these subjects:

  • Medical Oncology
  • Critical Care


Show Summary Details


Objective: The aim of this study was to analyse the spread of the blaOXA-58 gene as a source of carbapenem resistance in Acinetobacter baumannii in the burns unit of a university hospital in Toulouse in 2003–2004.

Methods: Six carbapenem-resistant A. baumannii isolates from six patients, and a carbapenem-resistant environmental A. baumannii isolate were collected in the burns unit of the Rangueil hospital (Toulouse). Susceptibility tests were carried out by disc diffusion and agar dilution methods. The detection of the blaOXA-58 gene was conducted by PCR followed by sequence analysis. Plasmids were extracted and hybridized with a probe specific for blaOXA-58. DNA fingerprints were obtained by pulsed-field gel electrophoresis of ApaI- or SmaI-digested chromosomal DNA of the tested strains.

Results: The multidrug-resistant clinical isolates had a similar 30 kb plasmid that encoded the carbapenem-hydrolysing β-lactamase OXA-58. These isolates were clonally related. The unrelated environmental carbapenem-resistant A. baumannii isolate had a similar blaOXA-58-carrying plasmid, suggesting spread of this gene.

Conclusions: A novel oxacillinase was the source of carbapenem resistance in the A. baumannii isolates. Its gene was plasmid-, but not integron-borne.

Keywords: A. baumannii; carbapenem resistance; nosocomial infections

Journal Article.  1895 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.