Journal Article

Enhanced resistance to erythromycin is conferred by the enterococcal <i>msrC</i> determinant in <i>Staphylococcus aureus</i>

Elinor Reynolds and Jonathan H. Cove

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 2, pages 260-264
Published in print February 2005 | ISSN: 0305-7453
Published online February 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh541
Enhanced resistance to erythromycin is conferred by the enterococcal msrC determinant in Staphylococcus aureus

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Objectives:

The msrC gene, found on the chromosome of Enterococcus faecium, shares a high degree of similarity with the staphylococcal erythromycin resistance determinant msr(A). The enterococcal determinant was cloned into Staphylococcus aureus to determine whether msrC could confer antibiotic resistance in staphylococci.

Methods:

A shuttle vector comprising pBluescript and pSK265 was used to introduce multiple copies of msrC into S. aureus RN4220. The integration vector pCL84 was employed to insert a single copy of msrC into the S. aureus chromosome. MICs were determined by the broth microdilution method.

Results:

Expression of msrC from both chromosomal and plasmid loci in erythromycin-susceptible S. aureus RN4220 (MIC 0.25 mg/L) gave rise to enhanced protection against erythromycin, with an MIC of 32–64 mg/L for S. aureus RN4220 containing msrC in multiple copies and an MIC of 16–64 mg/L with msrC inserted as a single copy in the S. aureus chromosome.

Conclusions:

MsrC mediates high-level resistance to erythromycin in S. aureus.

Keywords: macrolide resistance; ABC transporter; molecular cloning; staphylococci; enterococci

Journal Article.  2249 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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