Journal Article

<i>In vitro</i> activity and killing effect of temporin A on nosocomial isolates of <i>Enterococcus faecalis</i> and interactions with clinically used antibiotics

Andrea Giacometti, Oscar Cirioni, Wojciech Kamysz, Giuseppina D'Amato, Carmela Silvestri, Maria Simona Del Prete, Alberto Licci, Jerzy Łukasiak and Giorgio Scalise

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 2, pages 272-274
Published in print February 2005 | ISSN: 0305-7453
Published online February 2005 | e-ISSN: 1460-2091 | DOI: https://dx.doi.org/10.1093/jac/dkh545
In vitro activity and killing effect of temporin A on nosocomial isolates of Enterococcus faecalis and interactions with clinically used antibiotics

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Objective: To study the in vitro activity of temporin A, a basic, highly hydrophobic, antimicrobial peptide amide derived from the skin of the European red frog Rana temporaria, alone and in combination with co-amoxiclav, imipenem, ciprofloxacin, linezolid and vancomycin, against 42 nosocomial isolates of Enterococcus faecalis. Fourteen of these were resistant to vancomycin.

Methods: Antimicrobial activity of temporin A was measured by MIC, MBC and time–kill studies and by the chequerboard titration method.

Results: All isolates were inhibited at concentrations of 1 to 16 mg/L. Combination studies carried out with E. faecalis ATCC 29212 and ATCC 51299 demonstrated synergy only when the peptide was combined with co-amoxiclav and imipenem.

Conclusions: Our findings show that temporin A is active against E. faecalis and that its activity could be enhanced when it is combined with other antimicrobial agents.

Keywords: antimicrobial peptides; Gram-positive infections; susceptibility; antibiotic combinations; enterococci

Journal Article.  1847 words. 

Subjects: Medical Oncology ; Critical Care

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