Journal Article

Description of In116, the first <i>bla</i><sub>CTX-M-2</sub>-containing complex class 1 integron found in <i>Morganella morganii</i> isolates from Buenos Aires, Argentina

Pablo Power, Moreno Galleni, José Di Conza, Juan A. Ayala and Gabriel Gutkind

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 4, pages 461-465
Published in print April 2005 | ISSN: 0305-7453
Published online April 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkh556
Description of In116, the first blaCTX-M-2-containing complex class 1 integron found in Morganella morganii isolates from Buenos Aires, Argentina

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Objectives: We analysed the architecture and probable origin of a class 1 integron from cefotaxime-resistant Morganella morganii isolates.

Methods: bla genes and class 1 integron elements were detected by PCR and DNA–DNA hybridization in a M. morganii strain isolated in 1996. PCR-mapping and sequencing of different fragments were carried out to determine the integron's architecture.

Results and conclusions: A class 1 integron (In116), strongly related to the In6/In7 family, was detected in a plasmid from an oxyimino-cephalosporin-resistant M. morganii strain, producing CTX-M-2 β-lactamase. The variable region of In116 contains aacA4, blaOXA-2 and orfD cassettes. Downstream of the 3′-conserved-segment (3′-CS), an orf513-containing common region is followed by blaCTX-M-2 and flanking regions, having 96–99% nucleotide identity with Kluyvera ascorbata's kluA-1 and neighbouring sequences. Some of the evidence supporting the incorporation of foreign DNA is as follows: a partial deletion in a second 3′-CS (3′-CS2), and the absence of 59-base element or IS-like structures upstream of blaCTX-M-2.

Keywords: Kluyvera ascorbata; In6/In7; orf513; aacA4; bla OXA-2

Journal Article.  2583 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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