Journal Article

Resistance to ceftazidime is associated with a S220Y substitution in the omega loop of the AmpC β-lactamase of a <i>Serratia marcescens</i> clinical isolate

Nadia Hidri, Guilène Barnaud, Dominique Decré, Claude Cerceau, Valérie Lalande, Jean Claude Petit, Roger Labia and Guillaume Arlet

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 4, pages 496-499
Published in print April 2005 | ISSN: 0305-7453
Published online April 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki025
Resistance to ceftazidime is associated with a S220Y substitution in the omega loop of the AmpC β-lactamase of a Serratia marcescens clinical isolate

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Objectives: The aim of this study was to characterize the ampC β-lactamase gene of a clinical isolate of Serratia marcescens resistant to ceftazidime.

Methods: S. marcescens SMSA was isolated from an intra-abdominal wound of a patient previously treated with ceftazidime. A susceptible strain, SLS73, was used as a control. Susceptibility testing, PCR, DNA sequencing, molecular cloning, site-directed mutagenesis and determination of kinetic parameters were carried out to investigate the mechanism of resistance to ceftazidime.

Results: MICs of ceftazidime were 64 and 0.2 mg/L for SMSA and SLS73, respectively. Sequencing of the ampC gene of SMSA was carried out. When compared with the closest AmpC enzyme, the S. marcescens S3 β-lactamase, the novel protein showed E57Q, Q129K and S220Y substitutions. The S220Y substitution is located in the omega loop. Introduced by mutagenesis in the ampC gene of SLS73, this substitution conferred the same level of resistance to ceftazidime. The catalytic efficiency (kcat/Km) of the mutated enzyme toward ceftazidime was increased by about 100-fold.

Conclusions: We present another example of in vivo selection of broad-spectrum resistance by amino acid substitution in the omega loop of chromosomal AmpC β-lactamase in S. marcescens.

Keywords: extended-spectrum activities; mutagenesis; amino acid substitutions

Journal Article.  2065 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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