Journal Article

Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from <i>Shewanella livingstonensis</i> (SLB-1) and <i>Shewanella frigidimarina</i> (SFB-1)

Laurent Poirel, Claire Héritier and Patrice Nordmann

in Journal of Antimicrobial Chemotherapy

Volume 55, issue 5, pages 680-685
Published in print May 2005 | ISSN: 0305-7453
Published online May 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki065
Genetic and biochemical characterization of the chromosome-encoded class B β-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)

More Like This

Show all results sharing these subjects:

  • Medical Oncology
  • Critical Care

GO

Show Summary Details

Preview

Objectives: To determine the β-lactamase gene content of β-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment.

Methods: β-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes.

Results: Metallo-β-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B β-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum β-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.

Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of β-lactam resistance genes.

Keywords: metallo-β-lactamases; S. livingstonensis; S. frigidimarina; carbapenems

Journal Article.  3107 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.