Journal Article

Genetic analysis of <i>pbp2x</i> in clinical <i>Streptococcus pneumoniae</i> isolates in Quebec, Canada

Dominic Granger, Geneviève Boily-Larouche, Pierre Turgeon, Karl Weiss and Michel Roger

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 6, pages 832-839
Published in print June 2005 | ISSN: 0305-7453
Published online May 2005 | e-ISSN: 1460-2091 | DOI:
Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada

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Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations.

Methods: The pbp2x genomic region encompassing codons 178–703, which includes the entire region of the transpeptidase domain, was sequenced and compared for 52 clinical isolates comprising 20 penicillin-susceptible (PSSP), 20 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci.

Results: The degree of diversity within PBP2x correlated with increased resistance to β-lactam antibiotics. There were an average of 5.0 ± 1.8 mutations in PSSP, 37.9 ± 4.4 in PISP, and 63.0 ± 2.0 in PRSP isolates when compared with the control penicillin-susceptible strain R6. At least six distinct amino acid profiles were identified among PISP strains isolated in Quebec. In contrast, all PRSP isolates shared a similar pattern of altered amino acids compared with the sequence from susceptible strains.

Conclusions: These data will be useful in future studies to monitor the genetic changes associated with the emergence and spread of β-lactam resistance in Quebec.

Keywords: penicillin-binding proteins; β-lactams; pneumococci; serotype; penicillin resistance; cefotaxime resistance

Journal Article.  4370 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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