Journal Article

Gentamicin-loaded microspheres for treatment of experimental <i>Brucella abortus</i> infection in mice

Sandra Prior, Bruno Gander, Juan M. Irache and Carlos Gamazo

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 55, issue 6, pages 1032-1036
Published in print June 2005 | ISSN: 0305-7453
Published online June 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki144
Gentamicin-loaded microspheres for treatment of experimental Brucella abortus infection in mice

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Objectives: To evaluate the efficacy of gentamicin-loaded poly (lactide-co-glycolide) 50:50H (PLGA 50:50H) microspheres for the treatment of mice experimentally infected with Brucella abortus 2308.

Methods: The microspheres were dispersed in either 2% (w/v) poloxamer 188 saline solution, or deionized water with the help of a cell homogenizer to break up particle aggregates, and were administered intravenously or intraperitoneally to B. abortus-infected mice 7 days post-infection.

Results: Neither a single intravenous or intraperitoneal dose of 67 μg of gentamicin per mouse, nor three intraperitoneal doses of 100 μg of gentamicin per mouse, reduced the Brucella infection in the spleen compared with untreated mice 1 and 3 weeks post-treatment. Histological examination revealed granulation and tissue reaction in the periphery of spleen and liver of animals given three doses of the gentamicin-loaded microspheres.

Conclusions: The lack of therapeutic activity of the gentamicin-loaded microspheres might be related to inappropriate microsphere size and aggregation, resulting also in a poor distribution of the microspheres in the spleen. The results might provide an example of practical problems related to particle size and aggregation for in vivo therapy with PLGA microspheres.

Keywords: biodegradable microspheres; drug delivery systems; Brucella-infected mice

Journal Article.  2233 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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