Journal Article

Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of <i>Staphylococcus lugdunensis</i>

María Mateo, Juan-Ramón Maestre, Lorenzo Aguilar, Fabio Cafini, Pilar Puente, Paloma Sánchez, Luis Alou, María-José Giménez and José Prieto

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 2, pages 287-291
Published in print August 2005 | ISSN: 0305-7453
Published online July 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki227
Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of Staphylococcus lugdunensis

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Objectives: To compare different methods for the identification and determination of susceptibility to penicillin and methicillin of Staphylococcus lugdunensis.

Methods: Seventeen clinical isolates of S. lugdunensis (identified by PCR amplification and sequencing of the rpoB gene) were studied using the ATB32-Staph, Crystal, Vitek 2 and Wider commercial systems. The clumping factor test and the tube coagulase test were also performed. β-Lactamase production was studied by chromogenic methods. Methicillin resistance was phenotypically studied by the MRSA slide latex agglutination test, growth in MRSA agar, and the Vitek 2 and Wider systems (based on oxacillin MIC), and genotypically studied by detection of the mecA gene by PCR.

Results: The clumping factor test was negative in 35.3% of strains. All isolates were correctly identified to species level by the ATB32-Staph system. Species misidentification rates were 5.9%, 23.5% and 29.4% with the Crystal, the Vitek 2 and the Wider systems, respectively, mostly as Staphylococcus haemolyticus. β-Lactamase was present in 11.8% of strains. Whereas 76.5% and 47.1% of strains exhibited oxacillin resistance (MIC range 0.5–2 mg/L) by the Vitek 2 system and the Wider system, respectively, none of the strains was positive in the MRSA slide latex agglutination test or grew in MRSA agar. All strains lacked the mecA gene.

Conclusions: The clumping factor test and some commercial systems may misidentify S. lugdunensis. Oxacillin resistance detected by commercial systems is not indicative of the presence of the mecA gene. These facts, together with β-lactamase production, may preclude adequate treatment of infections by this virulent coagulase-negative Staphylococcus.

Keywords: S. lugdunensis; methicillin resistance; β-lactamases; mecA gene; clumping factor

Journal Article.  2800 words. 

Subjects: Medical Oncology ; Critical Care

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