Journal Article

Variation in selected regions of <i>bla</i><sub>TEM</sub> genes and promoters in <i>Haemophilus influenzae</i>

Stephen G. Tristram, Rebecca Hawes and Juliana Souprounov

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 3, pages 481-484
Published in print September 2005 | ISSN: 0305-7453
Published online July 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki238
Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae

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  • Medical Oncology
  • Critical Care

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Objectives: To determine if the TEM β-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.

Methods: Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the blaTEM genes of a collection of TEM-positive strains.

Results: All blaTEM genes were found to be blaTEM-1 or derivatives thereof and none blaTEM-2. The blaTEM genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.

Conclusions: The genetic features of blaTEM genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.

Keywords: H. influenzae; promoters; β-lactamases; blaTEM genes; TEM

Journal Article.  2765 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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