Journal Article

Prediction of resistance to erythromycin in the genus <i>Rickettsia</i> by mutations in L22 ribosomal protein

J. M. Rolain and D. Raoult

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 2, pages 396-398
Published in print August 2005 | ISSN: 0305-7453
Published online July 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki242
Prediction of resistance to erythromycin in the genus Rickettsia by mutations in L22 ribosomal protein

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Objectives: Typhus group (TG) rickettsiae are naturally susceptible to erythromycin whereas spotted fever group (SFG) rickettsioses are not. The aim of this study was to compare in silico genetic determinants known to be associated with resistance to macrolide compounds.

Methods and results: Available sequences of the 23S RNA gene, and L4 and L22 ribosomal proteins of rickettsial strains were aligned and compared using in silico methods. Although there were no sequence differences in domain V of the 23S RNA gene and in the conserved region of the L4 ribosomal protein gene, we found that TG rickettsiae had a triple amino acid difference in the highly conserved region of the L22 ribosomal protein compared with the SFG rickettsiae.

Conclusions: We believe that the triple amino acid difference in the L22 ribosomal protein found in this study may explain the difference in susceptibility to erythromycin among the Rickettsia genus. Genome analysis may help to predict possible molecular mechanisms of resistance for fastidious and intracellular bacteria and cloning and expression of such proteins should be investigated in the future in order to prove our hypothesis.

Keywords: antibiotic resistance; genome analysis; in silico; L22 protein; erythromycin resistance; macrolides

Journal Article.  1580 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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