Journal Article

Genetic basis for dissemination of <i>armA</i>

Bruno González-Zorn, Ana Catalan, Jose A. Escudero, Lucas Domínguez, Tirushet Teshager, Concepción Porrero and Miguel Angel Moreno

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 3, pages 583-585
Published in print September 2005 | ISSN: 0305-7453
Published online July 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki246
Genetic basis for dissemination of armA

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Objectives and methods: armA is a novel plasmid-borne 16S rRNA methyltransferase that confers high-level resistance to 4,6-disubstituted deoxystreptamines. Recently, we have isolated from a high-level broad-spectrum aminoglycoside-resistant Escherichia coli animal isolate a plasmid, pMUR050, that bore the armA gene. In order to elucidate the genetic basis for the spread of armA, we have determined the complete nucleotide sequence of pMUR050.

Results: armA was borne by a complex transposon composite flanked by two direct repeats of IS26. The transposon composite included a class one integron with sul1 for resistance to sulphonamides and ant3″9 conferring resistance to spectinomycin–streptomycin, and a macrolide efflux pump and mefE/mel conferring high-level resistance to erythromycin. We identified in GenBank that another plasmid, pCTX-M3, from a Polish Citrobacter freundii human isolate, bore the same genetic structure, including armA.

Conclusions: armA is present in human and animal isolates within a novel transposon composite. Further spread of armA between bacteria of diverse origin is to be expected.

Keywords: Escherichia coli; animal isolates; 16S rRNA methylase; aminoglycoside resistance; IncN; transposon composite

Journal Article.  1728 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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