Journal Article

Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of <i>Salmonella enterica</i> serotype Enteritidis in Hong Kong

Terence Kin Man Cheung, Yiu Wai Chu, Man Yu Chu, Choi Ha Ma, Raymond Wai Hung Yung and Kai Man Kam

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 3, pages 586-589
Published in print September 2005 | ISSN: 0305-7453
Published online July 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki250
Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype Enteritidis in Hong Kong

Show Summary Details

Preview

Objectives: To characterize the genetic determinants involved in the reduced susceptibility to ciprofloxacin and cefotaxime in Salmonella enterica serotype Enteritidis clinical isolates obtained from four patients in summer 2003 in Hong Kong.

Methods: Three Salmonella Enteritidis isolates from blood culture and one from stool were collected due to their increased resistance to ciprofloxacin and cefotaxime. PFGE analysis was used to investigate their genetic relatedness. Conjugation experiments were employed to show if the genetic determinants involved were plasmid-mediated. MICs of various antimicrobials were determined by VITEK 2 and Etest. Based on the susceptibility and conjugation experiment results, previously described PCR methods were employed to detect sequences homologous to qnr and blaCTX-M suspected to be involved in the reduced susceptibility to ciprofloxacin and cefotaxime, respectively.

Results: PFGE analysis showed that the four Salmonella isolates were clonally unrelated. The presence of a qnr-like gene and the CTX-M allele blaCTX-M-14 on four different transferable plasmids harboured by the four isolates was confirmed.

Conclusions: This is the first report of transferable fluoroquinolone resistance due to a new qnr allele, which appeared to be linked to blaCTX-M-14, in isolates of Salmonella Enteritidis in Hong Kong.

Keywords: Qnr; CTX-M-14; extended-spectrum β-lactamases; ESBLs

Journal Article.  2029 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.