Journal Article

Differences in the DNA sequence of the translational attenuator of several constitutively expressed <i>erm</i>(A) genes from clinical isolates of <i>Streptococcus agalactiae</i>

Esther Culebras, Iciar Rodríguez-Avial, Carmen Betriu and Juan J. Picazo

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 5, pages 836-840
Published in print November 2005 | ISSN: 0305-7453
Published online September 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki337
Differences in the DNA sequence of the translational attenuator of several constitutively expressed erm(A) genes from clinical isolates of Streptococcus agalactiae

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Objectives: To study the regulatory region of a constitutively expressed erm(A) gene in Streptococcus agalactiae clinical isolates.

Methods: Thirty clinical isolates of S. agalactiae which were cross-resistant to erythromycin and clindamycin and with a clindamycin MIC higher than that of erythromycin were studied by PCR, sequencing and molecular typing.

Results: PCR analysis revealed that all the strains harboured the erm(A) gene, either alone (26 isolates) or in combination with erm(B) (four isolates). Sequencing of the region upstream of erm(A) showed that all isolates possessed two types of genetic alteration. Most of the strains showed point mutations in the second leader peptide (mainly A137C) and, in four isolates (two clones), an insertion fragment with high homology to IS1381 and transposase genes was detected. Epidemiological analysis of strains indicated several clonal origins of isolates.

Conclusions: The mutations described here are thought to result in increased or constitutive expression of the erm(A) gene.

Keywords: clindamycin resistance; Group B streptococci; gene regulation

Journal Article.  2855 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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