Journal Article

Characterization of the highly variable region surrounding the <i>bla</i><sub>CTX-M-9</sub> gene in non-related <i>Escherichia coli</i> from Barcelona

Aurora García, Ferran Navarro, Elisenda Miró, Beatriz Mirelis, Susana Campoy and Pere Coll

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 56, issue 5, pages 819-826
Published in print November 2005 | ISSN: 0305-7453
Published online September 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki345
Characterization of the highly variable region surrounding  the blaCTX-M-9 gene in non-related Escherichia coli  from Barcelona

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Objectives: The dispersion of a clone, a plasmid or a mobile element carrying the blaCTX-M-9 gene was evaluated in 30 Escherichia coli strains isolated in Barcelona between 1996 and 1999. The presence of the previously described orf513-bearing class 1 integron, In60, carrying the blaCTX-M-9 gene, was also studied.

Methods: The clonality was analysed by pulsed-field gel electrophoresis. Plasmid analysis was performed by S1 digestion and hybridization with the CTX-M-9 probe. PCR mapping using specific designed primers was used to study the presence of In60 and In60-like structures.

Results: The clonality between the 30 strains was minor. The size of blaCTX-M-9 carrying plasmids ranged between ∼80 and 430 kb. One strain produced only a chromosome-encoded CTX-M-9 β-lactamase. Thirty-six per cent of the strains showed differences with respect to the In60 structure due to an insertion or deletion events.

Conclusions: These findings suggest that the bla CTX-M-9 gene may be carried by a mobile element that disperses it between plasmids. The fast dispersion of the CTX-M-9 enzyme could therefore be due to both diffusion of plasmids and mobile elements.

Keywords: β-lactamases; plasmids; integrons

Journal Article.  5314 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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