Journal Article

<i>In vitro</i> activity of a novel compound, the metal ion chelating agent AQ<sup>+</sup>, against clinical isolates of <i>Staphylococcus aureus</i>

Benjamin R. D. Short, Miguel A. Vargas, Jonathan C. Thomas, Simon O'Hanlon and Mark C. Enright

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 57, issue 1, pages 104-109
Published in print January 2006 | ISSN: 0305-7453
Published online November 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki428
In vitro activity of a novel compound, the metal ion chelating agent AQ+, against clinical isolates of Staphylococcus aureus

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  • Medical Oncology
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Objectives: To determine the efficacy of a novel antimicrobial compound, AQ+, against a genetically heterogeneous collection comprising 213 Staphylococcus aureus isolates from global sources. AQ+ is an aqueous preparation containing 0.5% 8-hydroxyquinoline.

Methods: MICs were found for all the isolates tested using the BSAC microdilution method. Time–kill studies were performed according to NCCLS guidelines. Transmission electron microscopy (TEM) was used to view the ultrastructural effects of AQ+.

Results: AQ+ was shown to strongly inhibit the growth of all isolates with a median MIC of 0.25% at a pH optimum of 9.2. Lowering the pH to 7.5 gave an ∼4-fold reduction in efficacy and at pH 5.5 there was an ∼8-fold reduction in efficacy. Methicillin-resistant S. aureus (MRSA) as well as vancomycin-intermediate S. aureus were shown to be as equally susceptible to AQ+ as methicillin-susceptible S. aureus. Time–kill curves for AQ+ were similar to those for gentamicin. TEM showed that AQ+ actively disrupts the cell wall of S. aureus leading to cell lysis.

Conclusions: These results suggest that AQ+ has strong antimicrobial activity and may be useful in preparations to reduce nasal and skin carriage of MRSA.

Keywords: 8-hydroxyquinoline; S. aureus; MRSA; time–kill; antimicrobial susceptibility

Journal Article.  3294 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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