Journal Article

Molecular characterization of methicillin-resistant <i>Staphylococcus aureus</i> strains from pet animals and their relationship to human isolates

Birgit Strommenger, Corinna Kehrenberg, Christiane Kettlitz, Christa Cuny, Jutta Verspohl, Wolfgang Witte and Stefan Schwarz

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 57, issue 3, pages 461-465
Published in print March 2006 | ISSN: 0305-7453
Published online December 2005 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki471
Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and their relationship to human isolates

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Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals were characterized and compared with human isolates from clonal complexes most prevalent in Central Europe.

Methods: S. aureus isolates were investigated for their in vitro susceptibility to antimicrobial agents by broth microdilution. Resistance genes and the Panton–Valentine leucocidin gene lukF-lukS were identified by PCR. All isolates were characterized by SmaI macrorestriction analysis and spa typing to assess their genomic relationships. Representative isolates were additionally analysed by multilocus sequence typing and PCR-directed SCCmec typing.

Results: All pet isolates carried the resistance genes mecA and erm(C) and proved to be resistant to β-lactams and MLSB antibiotics. In addition, all isolates were resistant to fluoroquinolones. None of the pet isolates carried the Panton–Valentine leucocidin gene lukF-lukS. Macrorestriction analysis revealed that the pet MRSA isolates exhibited four closely related SmaI fragment patterns. Moreover, all isolates revealed spa type t032. Further analysis of representatives of the different PFGE types revealed the presence of multilocus sequence type ST22 in combination with a type IV SCCmec element. Thus, molecular typing results were similar for pet strains and human ST22 reference strains.

Conclusions: Based on their strain characteristics, the MRSA isolates from pets investigated in this study closely resembled ST22 MRSA isolates which are widely disseminated in German hospitals. The results of this study indicate that cross-transmission of MRSA between pet animals and humans might have occurred.

Keywords: antimicrobial resistance; spa typing; MLST; SCCmec

Journal Article.  2495 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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