Journal Article

Distribution of a transposon-like element carrying <i>bla</i><sub>CMY-2</sub> among <i>Salmonella</i> and other Enterobacteriaceae

Lin-Hui Su, Hsiu-Ling Chen, Ju-Hsin Chia, Shu-Ying Liu, Chishih Chu, Tsu-Lan Wu and Cheng-Hsun Chiu

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 57, issue 3, pages 424-429
Published in print March 2006 | ISSN: 0305-7453
Published online January 2006 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dki478
Distribution of a transposon-like element carrying blaCMY-2 among Salmonella and other Enterobacteriaceae

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Objectives: The dissemination of cephamycin resistance in Enterobacteriaceae and its correlation with a transposon-like DNA element consisting of a specific tnpA-blaCMY-2-blc-sugE structure were investigated.

Methods: A total of 140 enterobacterial isolates belonging to 17 species (10 genera) of Enterobacteriaceae phenotypically characterized as putative AmpC-producers were evaluated. The isolates were examined by PCR analysis, DNA–DNA hybridization and nucleotide sequencing.

Results: The blaCMY-2-carrying element was detected in 34 isolates from 10 species (9 genera), including all 14 Salmonella and 4 Shigella isolates as well as 7 of the 10 Escherichia coli isolates tested. The remaining 9 isolates were from 112 isolates of the other 14 species tested. The genetic structure of the blaCMY-2-carrying element was identical in 29 isolates, while in 3 E. coli and 2 Citrobacter isolates an additional insertion sequence IS1 was found inserted at various nucleotide positions close to the 3′ end, either within or downstream, of tnpA. In 12 of the 14 representative isolates examined, the blaCMY-2-carrying element was found inserted in the finQ gene of various-sized plasmids with highly conserved 8 bp direct repeats flanking the junction regions. Among the other 106 non-CMY-2-producing isolates, plasmid-mediated ampC genes were found only in one isolate of Enterobacter aerogenes which carried a blaDHA-1-like gene.

Conclusions: blaCMY-2 is the most prevalent plasmid-mediated ampC gene among Enterobacteriaceae. All the blaCMY-2 genes identified in the present study were associated with a specific transposon-like element that may be responsible for the spread of blaCMY-2 among Enterobacteriaceae.

Keywords: plasmid-mediated AmpC β-lactamases; cephamycin resistance; DHA-1-like β-lactamases

Journal Article.  3672 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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