Journal Article

Resistance to a polyquaternium-1 lens care solution and isoelectric points of <i>Pseudomonas aeruginosa</i> strains

Gerda M. Bruinsma, Minie Rustema-Abbing, Henny C. van der Mei, Carol Lakkis and Henk J. Busscher

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 57, issue 4, pages 764-766
Published in print April 2006 | ISSN: 0305-7453
Published online January 2006 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkl011
Resistance to a polyquaternium-1 lens care solution and isoelectric points of Pseudomonas aeruginosa strains

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Objectives: The aim of this study was to correlate the cell surface hydrophobicity and charge of various strains of Pseudomonas aeruginosa with their resistance to a polyquaternium-1 lens care solution.

Methods: The 11 P. aeruginosa strains included were isolated from eyes, contact lenses, lens cases and lens care solutions. Cell surface hydrophobicities were determined from water contact angle measurements and surface charges were measured as a function of pH using particulate micro-electrophoresis.

Results: Strains resistant to polyquaternium-1 had an isoelectric point (IEP; pH where the bacterial zeta potential is zero) ranging from 4.0 to 5.5, whereas susceptible strains were more negatively charged than resistant strains and had an IEP between 1.3 and 1.9. Water contact angles ranged from hydrophilic (34°) to hydrophobic (124°), without showing a relation with antimicrobial resistance.

Conclusions: Results suggest that electrostatic repulsion between cationic molecules on the cell surface and quaternary ammonium compounds impedes the antimicrobial entering the cell.

Keywords: cationic molecules; quaternary ammonium compounds; hydrophobicity; surface charge

Journal Article.  1852 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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