Journal Article

Antifungal susceptibility of <i>Cryptococcus neoformans</i> and <i>Cryptococcus gattii</i> isolates from decayed wood of trunk hollows of <i>Ficus religiosa</i> and <i>Syzygium cumini</i> trees in north-western India

Zia U. Khan, Harbans S. Randhawa, Tusharantak Kowshik, Anuradha Chowdhary and Rachel Chandy

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 60, issue 2, pages 312-316
Published in print August 2007 | ISSN: 0305-7453
Published online June 2007 | e-ISSN: 1460-2091 | DOI: https://dx.doi.org/10.1093/jac/dkm192
Antifungal susceptibility of Cryptococcus neoformans and Cryptococcus gattii isolates from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees in north-western India

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Background

We present antifungal susceptibility data on environmental isolates of Cryptococcus neoformans (serotype A, n = 117) and Cryptococcus gattii (serotype B, n = 65) cultured from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees.

Methods

Susceptibilities to amphotericin B, fluconazole, ketoconazole, itraconazole and voriconazole were determined by using Etest. The MICs were read after 48 h as per the guidelines provided by the manufacturer.

Results

The MIC90s and susceptibility ranges for C. neoformans isolates were as follows: 0.094 (0.004–0.25) mg/L for amphotericin B, 4 (0.032–12) mg/L for fluconazole, 0.094 (0.004–0.75) mg/L for itraconazole, 0.064 (0.002–0.19) mg/L for ketoconazole, and 0.047 (0.006–0.125) mg/L for voriconazole, whereas for C. gattii isolates these were 0.125 (0.023–0.5) mg/L for amphotericin B, 8 (0.032–16) mg/L for fluconazole, 0.75 (0.006–2) mg/L for itraconazole, 0.125 (0.003–0.19) mg/L for ketoconazole, and 0.094 (0.004–0.125) mg/L for voriconazole. A comparison of the geometric means of MICs (mg/L) revealed that C. gattii was less susceptible than C. neoformans to amphotericin B (0.075 versus 0.051, P = 0.0003), fluconazole (2.912 versus 2.316, P = 0.003), itraconazole (0.198 versus 0.0344, P < 0.0001), ketoconazole (0.072 versus 0.037, P < 0.0001), and voriconazole (0.045 versus 0.023, P < 0.0001).

Conclusions

The antifungal susceptibility data obtained in this study indicate that the occurrence of primary resistance among environmental isolates of C. neoformans serotype A and C. gattii serotype B is rare, and serotype B isolates are less susceptible than serotype A isolates.

Keywords: Etest; fluconazole; itraconazole; voriconazole; amphotericin B

Journal Article.  2449 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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