Journal Article

Methicillin-resistant <i>Staphylococcus aureus</i> containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006

Wolfgang Witte, Birgit Strommenger, Christa Cuny, Dagmar Heuck and Ulrich Nuebel

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 60, issue 6, pages 1258-1263
Published in print December 2007 | ISSN: 0305-7453
Published online October 2007 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkm384
Methicillin-resistant Staphylococcus aureus containing the Panton-Valentine leucocidin gene in Germany in 2005 and 2006

Show Summary Details

Preview

Objectives

The aim of this paper is to attribute Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) to clonal lineages by molecular typing with special reference to isolates exhibiting spa type t008/multilocus sequence type (MLST) ST8 [widely disseminated in the USA as ‘community-associated MRSA (caMRSA) USA300’].

Methods

PVL-positive MRSA (n = 117) were detected among 4815 MRSA sent to the German National Reference Laboratory for typing. These isolates were analysed by PFGE, spa typing, multilocus sequence typing, grouping of SCCmec elements and PCR detection of arcA, msr(A), mph(B) and the ≥6 AT repeat unit in the SACOL0058 sequence.

Results

Among the 117 isolates, 80 exhibited spa type t044 (corresponding to MLST ST80) and 23 exhibited spa type t008/MLST ST8. Other spa types were sporadically represented. Further characterization of isolates exhibiting t008/ST8 by PCR [arcA, msr(A), mph(B), ≥6 AT repeat signature] indicates the arrival of caMRSA ‘USA300’ in Central Europe.

Conclusions

caMRSA ST80 still predominate; however, caMRSA ST8 exhibiting the characteristics of the ‘USA300’ clone became the second most frequent. Routine detection of this clone in clinical bacteriology can be easily performed by PCR.

Keywords: community MRSA; genotyping; molecular markers

Journal Article.  3192 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.