Journal Article

A novel functional class 2 integron in clinical <i>Proteus mirabilis</i> isolates

Quhao Wei, Qingfeng Hu, Shanshan Li, Huoyang Lu, Guoqiang Chen, Beiqiong Shen, Ping Zhang and Yonglie Zhou

in Journal of Antimicrobial Chemotherapy

Published on behalf of British Society for Antimicrobial Chemotherapy

Volume 69, issue 4, pages 973-976
Published in print April 2014 | ISSN: 0305-7453
Published online November 2013 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkt456
A novel functional class 2 integron in clinical Proteus mirabilis isolates

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  • Medical Oncology
  • Critical Care

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Objectives

To describe a novel functional class 2 integron that was found in clinical Proteus mirabilis isolates.

Methods

Class 1 and 2 integrons were screened by PCR in 153 clinical Proteus isolates. The variable regions of class 1 and 2 integrons were determined by restriction analysis and sequencing. The mutations of internal stop codons in class 2 integrons and their common promoters were also determined by sequencing. Enterobacterial repetitive intergenic consensus (ERIC)–PCR was used to analyse the phylogenetic relations of class 2 integron-positive P. mirabilis isolates.

Results

Class 1 integrons were detected in 96 (63%) of 153 Proteus isolates: eight different gene cassette arrays were detected, including dfrA32-ereA1-aadA2, which was detected for the first time in P. mirabilis. Class 2 integrons were detected in 101 (66%) of 153 Proteus isolates: four different gene cassette arrays were detected, including dfrA1-catB2-sat2-aadA1, which was detected for the first time in a class 2 integron. A novel functional class 2 integron was detected in 38 P. mirabilis isolates with a common promoter (−35 TTTAAT|16 bp|−10 TAAAGT). The variable region of this functional class 2 integron contained dfrA14 and three novel open reading frames with unknown functions. Very similar ERIC–PCR fingerprinting patterns were detected in these 38 P. mirabilis isolates and were different from other class 2 integron-positive isolates.

Conclusions

A novel functional class 2 integron was found for the first time in P. mirabilis. These functional class 2 integron-harbouring P. mirabilis isolates were likely to be clonally spread in our hospital.

Keywords: integrases; gene cassettes; horizontal gene transfer; recombination

Journal Article.  2171 words.  Illustrated.

Subjects: Medical Oncology ; Critical Care

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