Journal Article

Investigation of a possible outbreak of carbapenem-resistant <i>Acinetobacter baumannii</i> in Odense, Denmark using PFGE, MLST and whole-genome-based SNPs

Anette M. Hammerum, Frank Hansen, Marianne N. Skov, Marc Stegger, Paal S. Andersen, Anette Holm, Lotte Jakobsen and Ulrik S. Justesen

in Journal of Antimicrobial Chemotherapy

Volume 70, issue 7, pages 1965-1968
Published in print July 2015 | ISSN: 0305-7453
Published online March 2015 | e-ISSN: 1460-2091 | DOI: http://dx.doi.org/10.1093/jac/dkv072
Investigation of a possible outbreak of carbapenem-resistant Acinetobacter baumannii in Odense, Denmark using PFGE, MLST and whole-genome-based SNPs

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Objectives

The objectives were to study a possible outbreak of carbapenem-resistant Acinetobacter baumannii by comparing three different typing methods (PFGE, MLST and whole-genome SNPs) and to compare the resistance gene profiles of the isolates.

Methods

From December 2012 to October 2013, eight carbapenem-resistant A. baumannii were detected at Odense University Hospital, Odense, Denmark. These isolates were typed by PFGE, with ApaI and SmaI, respectively, and subjected to WGS. The WGS data were used for in silico extraction of MLST types using two different schemes, resistance genes and SNPs, to which 31 publicly available A. baumannii genomes were added.

Results

Using ApaI, the eight isolates had four different PFGE profiles, which were further differentiated using SmaI, separating one of the profiles into two distinct PFGE types. Five ST2 (Pasteur MLST) OXA-23-producing isolates, two ST1 OXA-72-producing isolates and one ST158 OXA-23-producing isolate were detected. The five ST2 isolates were subdivided into ST195, ST208 and ST218 using the Oxford MLST scheme. The phylogenetic analysis based on the core genome showed that six of the eight Danish A. baumannii isolates were located in three distinct clusters. The two remaining isolates did not cluster with other Danish or international isolates included in the study. Isolates that clustered using PFGE, Oxford MLST and phylogenetic analysis also shared similar resistance gene profiles.

Conclusions

The SNP profile, Oxford MLST, PFGE and resistance gene profiles clearly indicated spread of three different A. baumannii strains.

Keywords: antimicrobial resistance; antimicrobial resistance epidemiology; carbapenems

Journal Article.  2352 words. 

Subjects: Medical Oncology ; Critical Care

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