Journal Article

Comparison of HPLC and GC-MS for Measurement of Cocaine and Metabolites in Human Urine

Diane L. Phillips, Ian R. Tebbett and Roger L. Bertholf

in Journal of Analytical Toxicology

Volume 20, issue 5, pages 305-308
Published in print September 1996 | ISSN: 0146-4760
e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/20.5.305
Comparison of HPLC and GC-MS for Measurement of Cocaine and Metabolites in Human Urine

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High-performance liquid chromatography (HPLC) was compared with gas chromatography-mass spectrometry (GC-MS) for quantitation of cocaine, benzoylecgonine, norcocaine, and cocaethylene in urine. Calibration standards were prepared in human urine, and bupivacaine was added as the internal standard for quantitation. After solid-phase extraction, the reconstituted samples were divided into aliquots for analysis by HPLC and GC-MS. The analytical performance of the two methods were compared with regard to sensitivity, precision, and dynamic range. Results of GC-MS and HPLC analyses of nine urine specimens previously confirmed as positive for benzoylecgonine were compared. Analytical results by HPLC were comparable to GC-MS. Therefore, for many laboratories, HPLC is a useful alternative to GC-MS for measuring cocaine and metabolites in urine.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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