Journal Article

Determination of Ester-Type Local Anesthetic Drugs (Procaine, Tetracaine, and T-caine)in Human Serum by Wide-Bore Capillary Gas Chromatography with Nitrogen-Phosphorus Detection

Masaru Terada, Mohammed N. Islam, Zaw Tun, Katsuya Honda, Choei Wakasugi, Tatsuo Shinozuka, Jun-ichi Yanagida, Toshinori Yamamoto and Yukio Kuroiwa

in Journal of Analytical Toxicology

Volume 20, issue 5, pages 318-322
Published in print September 1996 | ISSN: 0146-4760
e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/20.5.318
Determination of Ester-Type Local Anesthetic Drugs (Procaine, Tetracaine, and T-caine)in Human Serum by Wide-Bore Capillary Gas Chromatography with Nitrogen-Phosphorus Detection

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A sensitive method for simultaneous determination of ester-type local anesthetic drugs (procaine, tetracaine, and T-caine) has been developed using wide-bore capillary gas chromatography with nitrogen-phosphorus detection (GC-NPD). The extraction procedure, the experimental conditions for heptafluorobutyryl (HFB) derivative formation, and the percentage of the ester-type local anesthetic drugs from the human serum are described. The HFB derivatives of ester-type local anesthetic drugs showed sensitivity of approximately 2-3-fold higher than that without derivatization. The detection limits of HFB derivatives of the ester-type local anesthetic drugs were approximately 60–70 pg on column. Recoveries from the human serum were 85–94%. This method could be used to determine concentrations as low as 24–28 ng/mL of the ester-type local anesthetic drugs.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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