Journal Article

Simultaneous Identification and Quantitation of Fluoxetine and its Metabolite, Norfluoxetine, in Biological Samples by GC-MS

Joseph A. Crifasi, Nha X. Le and Christopher Long

in Journal of Analytical Toxicology

Volume 21, issue 6, pages 415-419
Published in print October 1997 | ISSN: 0146-4760
Published online October 1997 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/21.6.415
Simultaneous Identification and Quantitation of Fluoxetine and its Metabolite, Norfluoxetine, in Biological Samples by GC-MS

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

A sensitive method for the quantitation of fluoxetine and norfluoxetine in biological samples was developed. Blood, urine, and tissue samples were alkalinized and extracted with N-butyl chloride. The extracts were derivatized with pentafluoropropionic anhydride before gas chromatography—mass spectrometry (GC-MS). Selected ions were monitored at m/z 117 and 294 for fluoxetine; m/z 117, 176, and 280 for norfluoxetine; and m/z 122 and 299 for the internal standard fluoxetine-d5. The within-run and between-run precision as well as recovery were determined for both analytes. The empirical limit of detection was determined to be 12.5 µg/L for both fluoxetine and norfluoxetine, whereas the empirical limit of quantitation was 25 µg/L for both drugs. Calibration curves were linear in the range of 50–1000 µg/L for both analytes. Some drugs that were known or suspected of interfering with high-performance liquid chromatography and GC methods for fluoxetine and norfluoxetine were tested for interference. This is the only reported method that combines the use of a deuterated internal standard, selected ion monitoring by GC-MS, and derivatization for the identification and quantitation of fluoxetine and norfluoxetine.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.