Journal Article

The Analysis of EDTA in Dried Bloodstains by Electrospray LC-MS-MS and Ion Chromatography*

Mark L. Miller, Bruce R. McCord, Roger Martz and Bruce Budowle

in Journal of Analytical Toxicology

Volume 21, issue 7, pages 521-528
Published in print November 1997 | ISSN: 0146-4760
Published online November 1997 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/21.7.521
The Analysis of EDTA in Dried Bloodstains by Electrospray LC-MS-MS and Ion Chromatography*

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

Analytical methods were developed to determine the presence of ethylenediaminetetraacetic acid (EDTA) in dried bloodstains to provide probative information when allegations of evidence tampering have been made in criminal cases. A simple screening method using ion chromatography to analyze stains was found to be quantitative to the 5 ppm level. The presence of EDTA was then confirmed using negative and positive ion mode liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods. A blind trial of these methods on 42 samples correctly determined the bloodstains that did and did not contain the preservative EDTA. One interesting observation in these results was the adsorption and postanalysis release of EDTA in the chromatographic system. In order to avoid cross contamination of samples resulting from this phenomena, it was found to be necessary to use EDTA-free blood extracts as blanks in the LC-MS analysis of bloodstains.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.