Journal Article

Determination of Methyl <i>tert</i>-Butyl Ether and <i>tert</i>-Butyl Alcohol in Human Urine by High-Temperature Purge-and-Trap Gas Chromatography-Mass Spectrometry

Chia-Wei Lee and Clifford P. Weisel

in Journal of Analytical Toxicology

Volume 22, issue 1, pages 1-5
Published in print January 1998 | ISSN: 0146-4760
Published online January 1998 | e-ISSN: 1945-2403 | DOI:
Determination of Methyl tert-Butyl Ether and tert-Butyl Alcohol in Human Urine by High-Temperature Purge-and-Trap Gas Chromatography-Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)


Show Summary Details


Methyl tert-butyl ether (MTBE) is the oxygenated gasoline additive most widely used in the U.S. to reduce the CO emission from motor vehicles. We developed a method using a high-temperature purge-and-trap procedure coupled with capillary gas chromatography-mass selective detection to determine MTBE and its metabolite, tert-butyl alcohol (TBA), in human urine. Several spiked-urine tests were conducted at different purging temperatures (25, 55, and 90°C). The results indicated that the purging temperature affects the recovery of TBA from urine more than the recovery of MTBE. The mean recoveries of MTBE and TBA in the urine samples by the high temperature (90°C) purge-and-trap gas chromatography-mass spectrometry method at different spike levels were 96.5 ± 4.7% and 98.4 ± 5.7%, respectively. The method was used to evaluate the urinary levels in a single subject exposed through inhalation to 1 ppm MTBE for 10 rain in a controlled-environment facility. Increases in MTBE and TBA urinary excretion rates were clearly evident following the exposure to MTBE. Approximately 0.9% of the amount of MTBE inhaled was excreted unchanged as urinary MTBE, and 2.4% was excreted as urinary TBA within 10 h after exposure. The method developed is a simple, effective, sensitive, and reproducible procedure for evaluating human exposure to MTBE.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.