Journal Article

A New Specific Method to Detect Cyanide in Body Fluids, Especially Whole Blood, by Fluorimetry

D. Felscher and M. Wulfmeyer

in Journal of Analytical Toxicology

Volume 22, issue 5, pages 363-366
Published in print September 1998 | ISSN: 0146-4760
Published online September 1998 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/22.5.363
A New Specific Method to Detect Cyanide in Body Fluids, Especially Whole Blood, by Fluorimetry

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This study shows a simple, rapid, and specific method for the quantitative determination of cyanide ion in body fluids, especially blood, by fluorimetry. It is based upon the transformation of cyanide ion into hydrocyanic acid, which then reacts with 2,3-naphthalenedialdehyde and taurine in a self-contained system. The 1-cyano-2-benzoisoindole derivate thus formed is suitable for fluorimetric measurement (λEX = 418 nm; λEM = 460 nm). The fluorescence intensity can be determined by spectrophotometry or by high-performance liquid chromatography (HPLC) with fluorescence detection. The detection limit is 0.002 pg/mL. Linearity was excellent from 0.002 to 1 µg/ml, for spectrophotometry and from 0.002 to 5 µg/mL for HPLC with fluorescence detection. The coefficient of variation for repeatability was 8% or less. Thiocyanate and sulfide did not interfere, even at high concentrations (200 µg/mL). The method was applicable to whole blood, so it should be suitable for both clinical and forensic purposes.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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