Journal Article

Evaluation of the Diagnostic Performance of the Boehringer Mannheim CEDIA<sup>®</sup> LSD Assay*

Alain G. Verstraete and Sophia Steyaert

in Journal of Analytical Toxicology

Volume 22, issue 7, pages 601-604
Published in print November 1998 | ISSN: 0146-4760
Published online November 1998 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/22.7.601
Evaluation of the Diagnostic Performance of the Boehringer Mannheim CEDIA® LSD Assay*

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The precision and the diagnostic performance of the Boehringer Mannheim CEDIA DAU LSD assay was evaluated. The assay was performed in the semi-quantitative mode on a Hitachi 917 analyzer. Within-run coefficients of variation (CVs) of the semiquantitative values for 0.25 and 1.0 ng/mL were 11.2 and 6.2%, respectively. Day-to-day CVs for the same concentrations were 12.6 and 8.6%. We analyzed 318 urine samples by CEDIA, DPC® Coat-A-Count® RIA and Behring EMIT®II. Confirmation was performed by GC-MS, after extraction on Bond Elut Certify® columns. Two hundred sixty-three samples were negative by all methods. Twenty-five samples were positive by all immunoassays, 19 of which were confirmed by gas chromatography-mass spectrometry (GC-MS). One sample was falsely negative by CEDIA. Three samples were positive by EMIT and CEDIA, but negative by RIA and GC-MS. Twenty-six samples were positive by EMIT alone, but they were not confirmed by GC-MS. The LSD CEDIA assay seems to be less specific than DPC RIA but more specific than the EMIT LSD assay.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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