Journal Article

Another Use of Silica Gel and Aqueous Eluent for HPLC Analysis of Clozapine and Desmethylclozapine

François Coudoré, Alain Nicolay, Monique Hoareau and Alain Eschalier

in Journal of Analytical Toxicology

Volume 23, issue 3, pages 195-199
Published in print May 1999 | ISSN: 0146-4760
Published online May 1999 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/23.3.195
Another Use of Silica Gel and Aqueous Eluent for HPLC Analysis of Clozapine and Desmethylclozapine

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

A procedure that involves a high-performance liquid chromatographic system with silica-bonded columns and reversed-phase eluents was fitted from a previously described method to measure clozapine and desmethylclozapine plasma levels. Clozapine and its demethylated metabolite were extracted from alkalinized serum by a liquid-liquid extraction, separated in 10 min, then quantitated at 254 nm at a minimum concentration of 20 ng/mL. The standard curves were linear over the range of 50–3000 ng/mL (r > 0.99) both for clozapine and desmethylclozapine and the assay had good sensitivity and recovery. Intra- and interday coefficients of variation for 200 and 800 ng/mL controls were less than 11.5% for clozapine and desmethylclozapine. This simple and efficient assay was used to monitor clozapine and desmethylclozapine levels from some treatment-refractory schizophrenic patients.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.