Journal Article

Determination by <sup>1</sup>H NMR Spectroscopy of Paraquat in Urine from Acutely Poisoned Patients. Comparison with Second-Derivative Spectroscopy Method

Michel Imbenotte, Nathalie Azaroual, Daniel Mathieu, Bernard Cartigny, Gaston Vermeersch and Michel Lhermitte

in Journal of Analytical Toxicology

Volume 23, issue 7, pages 586-590
Published in print November 1999 | ISSN: 0146-4760
Published online November 1999 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/23.7.586
Determination by 1H NMR Spectroscopy of Paraquat in Urine from Acutely Poisoned Patients. Comparison with Second-Derivative Spectroscopy Method

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

The application of 1H nuclear magnetic resonance (NMR) spectroscopy to characterize and quantitate paraquat in urine is described. Characterization was performed taking advantage of two NMR spectroscopy parameters: chemical shifts and coupling patterns. Without any pretreatment of the biological samples, herbicide was detected by its aromatic doublets at 8.49 and 9.02 ppm. Quantitation of the xenobiotic was realized by relative integration of the dipyridyl protons to an internal standard. After a validation step using control urine samples, quantitation was performed in urine obtained from two poisoned patients. On admission, mean paraquat concentrations were 985 (patient 1) and 500 (patient 2) µmol/L. Results are compared and found to be in good agreement, using a second-derivative spectroscopy method.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.