Journal Article

On-Line Immunoaffinity Extraction and HPLC Analysis of Flunitrazepam and Its Main Metabolites in Serum

I. Deinl, C. Franzelius, L. Angermaier, G. Mahr and G. Machbert

in Journal of Analytical Toxicology

Volume 23, issue 7, pages 598-602
Published in print November 1999 | ISSN: 0146-4760
Published online November 1999 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/23.7.598
On-Line Immunoaffinity Extraction and HPLC Analysis of Flunitrazepam and Its Main Metabolites in Serum

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A sensitive, simple, and rapid method for the determination of flunitrazepam and its major metabolites (7-aminoflunitrazepam, 7-acetamidoflunitrazepam, and norflunitrazepam) in serum and plasma is presented. The on-line procedure uses an immobilized, highly reusable antibody against benzodiazepines for selective extraction from serum followed by analysis by high-performance liquid chromatography with ultraviolet detection. This reliable method provides a limit of detection of 1 ng/mL serum, and results are obtained in less than 40 min.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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