Journal Article

Rapid Extraction of Clenbuterol from Human and Calf Urine Using Empore™ C8 Extraction Disks

A. Koole, A.C. Jetten, Y. Luo, J.P. Franke and R.A. de Zeeuw

in Journal of Analytical Toxicology

Volume 23, issue 7, pages 632-365
Published in print November 1999 | ISSN: 0146-4760
Published online November 1999 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/23.7.632
Rapid Extraction of Clenbuterol from Human and Calf Urine Using Empore™ C8 Extraction Disks

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In the present paper, disk extraction was evaluated for the rapid isolation of clenbuterol from human and calf urine, followed by high-performance liquid chromatography analysis with UV detection. A method was developed for the extraction with standard density C8 disks. The disks could be washed with 25% methanol in 0.01M sodium hydroxide without significant losses of clenbuterol. The recovery of clenbuterol was about 85%, and the extracts were clean. The detection limit was about 10 ng/mL. The main advantages of these disks were the saving of time and the reduced amounts of organic solvents needed.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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