Journal Article

Use of Solid-Phase Microextraction (SPME) for the Determination of Methadone and Its Main Metabolite, EDDP, in Plasma by Gas Chromatography-Mass Spectrometry

A.M. Bermejo, R. Seara, A.C. dos Santos Lucas, M.J. Tabernero, P. Fernández and R. Marsili

in Journal of Analytical Toxicology

Volume 24, issue 1, pages 66-69
Published in print January 2000 | ISSN: 0146-4760
Published online January 2000 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/24.1.66
Use of Solid-Phase Microextraction (SPME) for the Determination of Methadone and Its Main Metabolite, EDDP, in Plasma by Gas Chromatography-Mass Spectrometry

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A simple, rapid method for the determination of methadone and its metabofite 2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry is proposed. A 100-µm polydimethylsiloxane film fiber was exposed by immersion for 30 min in a diluted plasma solution (1:4 with buffer pH 9) containing both compounds and an internal standard (proadifen). Calibration curves were linear over the concentration range 50–2000 ng/mL. The analysis time was 45 min per sample. The determination of methadone and EDDP was subject to no interference. The performance of SPME was compared with that of liquid-liquid extraction, obtaining lower limits of detection for EDDP. The method using the two extraction procedures was applied to 10 plasma samples from methadone-treated patients.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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