Journal Article

GCD Quantitation of Opiates as Propionyl Derivatives in Blood

Sirpa Mykkänen, Janne Seppälä, Kari Ariniemi and Pirjo Lillsunde

in Journal of Analytical Toxicology

Volume 24, issue 2, pages 122-126
Published in print March 2000 | ISSN: 0146-4760
Published online March 2000 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/24.2.122
GCD Quantitation of Opiates as Propionyl Derivatives in Blood

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We describe a method using a gas chromatograph with electron ionization detection (GCD) for the simultaneous determination of morphine, codeine, 6-monoacetylmorphine, ethylmorphine, and dihydrocodeine in blood. The method employs propionic anhydride in the presence of triethylamine to propionylate free hydroxyl groups of the opiates in blood. The quantitation is achieved by using GCD with selected ion monitoring of the two most characteristic ions for each analyte. The quantitation limit was 0.01 mg/L and the linearity was 0.01–10 mg/L for dihydrocodeine, ethylmorphine, and 6-monoacetylmorphine. For the other investigated opiates, the quantitation limit was 0.025 mg/L and linearity was 0.025–10 mg/L. The intraday relative standard deviation (RSD) varied from 7.2 to 10% at the 0.5 mg/L level, and the day-to-day RSDs varied from 7.5 to 11% at the 0.85 mg/L level.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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