Journal Article

Discrimination of Dimethylamphetamine and Methamphetamine Use: Simultaneous Determination of Dimethylamphetamine-<i>N</i>-Oxide and Other Metabolites in Urine by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

Munehiro Katagi, Michiaki Tatsuno, Akihiro Miki, Mayumi Nishikawa and Hitoshi Tsuchihashi

in Journal of Analytical Toxicology

Volume 24, issue 5, pages 354-358
Published in print July 2000 | ISSN: 0146-4760
Published online July 2000 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/24.5.354
Discrimination of Dimethylamphetamine and Methamphetamine Use: Simultaneous Determination of Dimethylamphetamine-N-Oxide and Other Metabolites in Urine by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

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A simple and sensitive method by high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been investigated for the simultaneous determination of dimethylamphetamine (DMA), its specific yet labile main metabolite dimethylamphetamine-N-oxide (DMAO), and other metabolites, methamphetamine (MA) and amphetamine (AP), in urine. A combination of Bond Elut SCX columns for the solid-phase extraction of urine and a semi-micro SCX column for LC separations provided satisfactory results. The use of acetonitrile/5mM ammonium acetate buffer adjusted to pH 4 (65:35, v/v) as the mobile phase at a flow rate of 0.2 mL/min was found to be the most effective. The detection limits were 5 ng/mL for DMAO, 10 ng/mL for DMA and MA, and 50 ng/mL for AP in the SIM mode.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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