Journal Article

GC-MS Analysis of the Total Δ<sup>9</sup>-THC Content of Both Drug- and Fiber-Type Cannabis Seeds

Samir A. Ross, Zlatko Mehmedic, Timothy P. Murphy and Mahmoud A. ElSohly

in Journal of Analytical Toxicology

Volume 24, issue 8, pages 715-717
Published in print November 2000 | ISSN: 0146-4760
Published online November 2000 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/24.8.715
GC-MS Analysis of the Total Δ9-THC Content of Both Drug- and Fiber-Type Cannabis Seeds

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A GC-MS method was performed to determine the total Δ9-THC content in both drug- and fiber-type cannabis seeds. Drug-type seeds were found to contain much higher levels of Δ9-THC (35.6–124 µg/g) than fiber (hemp) seeds (0–12 µg/g). The majority of Δ9-THC was found to be located on the surface of the seeds. Approximately 90% of the total Δ9-THC was removed by a simple, quick wash with chloroform. Washed drug-type seeds contained less than 10 µg/g. Separation of the seeds into the kernel and testa showed that the bulk of Δ9-THC is located in the testa, mainly on the outside. The kernels of drug- and fiber-type cannabis seeds contained less than 2 and 0.5 µg Δ9-THC/g seeds, respectively. Fluctuations in the Δ9-THC content of different replicates of the same type of seeds could be the result of the degree of contamination on the outside of the seeds.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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