Journal Article

Simultaneous Determination of 2,4-D and MCPA in Canine Plasma and Urine by HPLC with Fluorescence Detection Using 9-Anthryldiazomethane (ADAM)

Laurene M. Dickow, Diane F. Gerken, Richard A. Sams and Susan M. Ashcraft

in Journal of Analytical Toxicology

Volume 25, issue 1, pages 35-39
Published in print January 2001 | ISSN: 0146-4760
Published online January 2001 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/25.1.35
Simultaneous Determination of 2,4-D and MCPA in Canine Plasma and Urine by HPLC with Fluorescence Detection Using 9-Anthryldiazomethane (ADAM)

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A method for the simultaneous determination of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-methyl-4-chlorophenoxyacetic acid (MCPA) in canine plasma and urine has been developed. This method used derivatization of extracted samples with 9-anthrylmethane (ADAM) for analysis by reversed-phase high-performance liquid chromatography with fluorescence detection. Precision and accuracy were within the accepted limits of 15% and 85–115%, respectively, for both analytes in plasma and urine. Calibration curves for 2,4-D and MCPA in plasma were linear (r2 > 0.99) between 0.50 and 5.0 mg/L and 5.0 and 100 mg/L. Calibration curves for 2,4-D and MCPA in urine were linear (r2 > 0.99) between 5.0 and 70.0 mg 2,4-D/L and 10.0 and 70.0 mg MCPA/L. The lower limit of detection was 62.5 ng/mL for both 2,4-D and MCPA.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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