Journal Article

Determination of Glufosinate Ammonium and its Metabolite, 3-Methylphosphinicopropionic Acid, in Human Serum by Gas Chromatography-Mass Spectrometry following Mixed-Mode Solid-Phase Extraction and <i>t</i>-BDMS Derivatization

Yasushi Hori, Manami Fujisawa, Kenji Shimada and Yasuo Hirose

in Journal of Analytical Toxicology

Volume 25, issue 8, pages 680-684
Published in print November 2001 | ISSN: 0146-4760
Published online November 2001 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/25.8.680
Determination of Glufosinate Ammonium and its Metabolite, 3-Methylphosphinicopropionic Acid, in Human Serum by Gas Chromatography-Mass Spectrometry following Mixed-Mode Solid-Phase Extraction and t-BDMS Derivatization

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A method for the analysis of glufosinate ammonium (GLUF) and its metabolite 3-methylphosphinicopropionic acid (MPPA) in human serum by gas chromatography-mass spectrometry (GC-MS) was developed. Employing a mixed-mode cartridge with both anion exchange action and weak nonpolar interaction, we extracted GLUF and MPPA from the serum and carried out GC-MS analysis of their tert-butyldimethylsilyl derivatives. The detection limits of GLUF and MPPA were 10 pg and 1 pg, respectively. Full mass spectra of 100 pg GLUF and of 10 pg MPPA were easily obtainable. The recovery rate of 90.0 ± 11.9% (or better) when the serum concentrations of GLUF and MPPA were 10-0.1 µg/mL. Results of 23 serum samples, from patients with GLUF poisoning, measured by this method correlate well with those derived from the conventional high-performance liquid chromatography method (r = 0.996). The developed GC-MS method is likely to become a useful analytical technique in clinical settings.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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