Journal Article

Human Nutritional Supplements in the Horse. Dehydroepiandrosterone versus Androstenedione: Comparative Effects on the Androgen Profile and Consequences for Doping Analysis

L. Dehennin, Y. Bonnaire and P. Plou

in Journal of Analytical Toxicology

Volume 25, issue 8, pages 685-690
Published in print November 2001 | ISSN: 0146-4760
Published online November 2001 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/25.8.685
Human Nutritional Supplements in the Horse. Dehydroepiandrosterone versus Androstenedione: Comparative Effects on the Androgen Profile and Consequences for Doping Analysis

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

Dehydroepiandrosterone (DHEA) and androstenedione are weak androgens, which need conversion to more potent testosterone in order to enhance anabolic action. Consequences of oral dosing at 1 mg/kg on the urinary and plasma androgen profile of mare and gelding have been evaluated with an analytical method involving conjugate fractionation and selective hydrolysis, group separation, and quantitation by gas chromatography-mass spectrometry with selected ion monitoring of trimethylsilyl ethers. Peak levels of testosterone total conjugates in urine (range 300–6000 µg/L) were attained a few hours after dosing. Renal clearance was fast, so the testosterone detection period lasted only 20 to 33 h, the longest time being generated by androstenedione. The urinary testosterone/epitestosterone ratio for detection of exogenous testosterone in the mare was inoperative after DHEA administration because there was a concomitant increase of epitestosterone, which thereby acted as a masking agent. Androstanediols and androstenediols, as well as some 17-ketosteroids, were additional markers. A transient increase of circulating free testosterone has been evidenced, and this would support possible anabolic/androgenic action by supplementation with DHEA and androstenedione along the oral route.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.