Journal Article

Simple Method of Methylation for Gas Chromatographic Analysis of <i>S</i>-Benzyl-<i>N</i>-acetylcysteine, a Metabolite of Toluene, in Human Urine

Setsunori Takahashi, Takashi Uezono, Atsushi Akane and Kojiro Kimura

in Journal of Analytical Toxicology

Volume 26, issue 4, pages 189-192
Published in print May 2002 | ISSN: 0146-4760
Published online May 2002 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/26.4.189
Simple Method of Methylation for Gas Chromatographic Analysis of S-Benzyl-N-acetylcysteine, a Metabolite of Toluene, in Human Urine

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A simplified method of methylation for the determination of urinary S-benzyl-N-acetylcysteine (benzylmercapturic acid, SBAC), a metabolite of toluene, by gas chromatography (GC) was developed. Acidified urine samples (pH 2) were extracted once with ethyl acetate and then derivatized to methyl ester (ME) using HCI-methanol. The optimum conditions for derivatization for SBAC and the internal standard (S-phenethyl-N-acetylcysteine) were reaction at 60°C for 20 rain. The SBAC-ME was measured by capillary GC. The calibration curve showed good linearity over the range of 0.2 to 5.0 mg/L (r = 0.986). This method was compared with a previously developed diazomethane methylation method for testing urine from subjects who had sniffed toluene. The values obtained by the two different methods were in good accordance. These results suggest that this technique for the methylation of SBAC by means of HCI-methanol is simpler and time-saving, thus making it feasible to determine SBAC and other mercapturic acids in urinary samples obtained from subjects who have been exposed to organic solvents.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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