Journal Article

A General Screening Method for Acidic, Neutral, and Basic Drugs in Whole Blood using the Oasis MCX<sup>®</sup> Column

J. Yawney, S. Treacy, K.W. Hindmarsh and F.J. Burczynski

in Journal of Analytical Toxicology

Volume 26, issue 6, pages 325-332
Published in print September 2002 | ISSN: 0146-4760
Published online September 2002 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/26.6.325
A General Screening Method for Acidic, Neutral, and Basic Drugs in Whole Blood using the Oasis MCX® Column

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Solid-phase extraction (SPE) is becoming a commonly used extraction technique. Most existing SPE methods extract a single drug from a relatively clean biological matrix (e.g., plasma, serum, or urine) using a silica-based column. These methods, however, are generally not satisfactory for forensic applications because the majority of biological samples are not as clean (e.g., whole blood, bile, tissues). Silica-based columns also may have reproducibility and stability problems. Polymer-based columns have been developed to overcome some of these limitations. In this study, sequential extraction of acidic, neutral, and basic drugs from whole blood using a polymer-based column, Oasis MCX, was undertaken. The extraction procedure developed involved a conditioning step using methanol followed by water; a three-step wash sequence using water, 0.1M hydrochloric acid, then water/methanol (95:5); and two elution steps. One elution step was for acidic and neutral drugs utilizing acetone/chloroform (1:1), and a second used ethyl acetate/ammonium hydroxide (98:2) for basic drugs. Of the drugs tested, 75% were extractable from whole blood and detectable at therapeutic concentrations. Good recoveries and clean extracts were achieved for the basic drugs; however, the extracts were not as clean for acidic drugs. Unfortunately, the Oasis MCX procedure was not suitable for extracting all drugs (e.g., benzodiazepines).

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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