Journal Article

Quantitative Analysis of Selegiline and Three Metabolites (<i>N</i>-Desmethylselegiline, Methamphetamine, and Amphetamine) in Human Plasma by High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Tandem Mass Spectrometry

Matthew H. Slawson, James L. Taccogno, Rodger L. Foltz and David E. Moody

in Journal of Analytical Toxicology

Volume 26, issue 7, pages 430-437
Published in print October 2002 | ISSN: 0146-4760
Published online October 2002 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/26.7.430
Quantitative Analysis of Selegiline and Three Metabolites (N-Desmethylselegiline, Methamphetamine, and Amphetamine) in Human Plasma by High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Tandem Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

This report describes a sensitive and specific high-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry method for the detection of subnanogram concentrations of selegiline and its three principle metabolites, N-desmethylselegiline, methamphetamine, and amphetamine, in human plasma. The assay has a dynamic range of 0.1–20 ng/mL for selegiline and N-desmethylselegiline (norselegiline) and 0.2–20 ng/mL for methamphetamine and amphetamine. The inter- and intra-assay precision and accuracy varied by less than 11% for all analytes at 0.3, 2.5, and 15 ng/mL and less than 16% at the lower limit of quantitation (0.1 ng/ml, for selegiline and norselegiline; and 0.2 ng/mL for methamphetamine and amphetamine). Selegiline and its metabolites showed no significant loss in quantitative accuracy after three freeze/thaw cycles or after up to 6 h at room temperature prior to extraction. Extracted plasma samples retained quantitative accuracy after storage for at least 7 days at −20°C or up to 70 h at room temperature. Methanolic stock solutions were stable for at least 6 h when kept at room temperature or at least 90 days when kept at −20°C.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.