Journal Article

Determination of Δ<sup>9</sup>-THC in Whole Blood using Gas Chromatography-Mass Spectrometry

Mark Hok Chi Chu and Olaf H. Drummer

in Journal of Analytical Toxicology

Volume 26, issue 8, pages 575-581
Published in print November 2002 | ISSN: 0146-4760
Published online November 2002 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/26.8.575
Determination of Δ9-THC in Whole Blood using Gas Chromatography-Mass Spectrometry

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A simple and reliable liquid-liquid extraction method for the determination of Δ9-tetrahydrocannabinol (THC) in whole blood utilizing gas chromatography-mass spectrometry in electron impact mode is described. The substance is derivatized with pentafluoropropionic anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL specimen, with recovery greater than 70%. The intra-assay coefficient of variation (CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC concentrations of 1, 5, and 25 ng/mL. The accuracy is between 95 and 97%. The optimization of extraction and derivatization conditions is detailed.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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