Journal Article

Determination of the Herbicide Glyphosate and its Metabolite in Biological Specimens by Gas chromatography-mass Spectrometry. A Case of Poisoning by Roundup<sup>®</sup> Herbicide

Yasushi Hori, Manami Fujisawa, Kenji Shimada and Yasuo Hirose

in Journal of Analytical Toxicology

Volume 27, issue 3, pages 162-166
Published in print April 2003 | ISSN: 0146-4760
Published online April 2003 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/27.3.162
Determination of the Herbicide Glyphosate and its Metabolite in Biological Specimens by Gas chromatography-mass Spectrometry. A Case of Poisoning by Roundup® Herbicide

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

In Japan, poisonings by the glyphosate (GLYP)-containing herbicide Roundup and the gluphosinate (GLUF)-based herbicide BASTA® have been increasing since about 1987. We applied the gas chromatography-mass spectrometry (GC-MS) method of analysis, on which we have already reported in regard to the determination of the blood serum level of GLUF and its melabolite, for the determination of serum and urinary levels of GLYP and its metabolite aminomethyl phosphonic acid (AMPA). Derivatization using N-methyl-N-(tert-butyldimethylsilyl) trifluoroacetamide was completed at a temperature of 80°C after 30 rain, and the detection limit of GLYP was 10 pg using m/z 454 and that of AMPA was 1 pg using m/z 396. The full mass spectra of 100 pg GLYP and of 10 pg AMPA were obtained easily. In extractions for which the Isolute® HAX cartridge was employed, the mean recovery rate of GLYP and AMPA added to serum to yield concentrations of 10-0.1 µg/mL (n = 5) was 91.6 ± 10.6% (or better), whereas that of GLYP and AMPA added to urine to yield concentrations of 100-1.0 µp/mL (n = 10) was 93.3 ± 6.6% (or better), both of which were good rates. Also, using this method of analysis, the presence of GLYP was identified in the full mass spectra obtained from the serum of a patient who may or may not have ingested Roundup.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.