Journal Article

Quantitation of Cocaine and Its Major Metabolites in Human Saliva Using Gas Chromatography-Positive Chemical Ionization-Mass Spectrometry (GC-PCI-MS)*

P. Cámpora, A.M. Bermejo, M.J. Tabernero and P. Fernández

in Journal of Analytical Toxicology

Volume 27, issue 5, pages 270-274
Published in print July 2003 | ISSN: 0146-4760
Published online July 2003 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/27.5.270
Quantitation of Cocaine and Its Major Metabolites in Human Saliva Using Gas Chromatography-Positive Chemical Ionization-Mass Spectrometry (GC-PCI-MS)*

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An analytical method for the simultaneous determination of cocaine (COC) and its major metabolites, ecgonine methyl ester (EME) and benzoylecgonine (BEG), in saliva was developed. The method involves liquid-liquid extraction in Toxitubes A®, derivatization with 99:1 (v/v) N,O-bis-trimelhylsilyltrifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS) and gas chromatography-mass spectrometry (GC-MS) determination. The detector response thus obtained is linear over the range 25–1000 ng/mL, with a reproducibility better than 93% and a recovery close to 100% for the three analytes. The limits of detection achieved were 0.9 ng/mL for EME, 2.2 ng/mL for cocaine, and 0.2 ng/mL for BEG, and the limits of quantilation were 3.0 ng/mL for EME, 7.4 ng/mL for cocaine, and 0.8 ng/m/for BEG. The proposed method was applied to 48 saliva samples from cocaine users, 96% of which were positive for the drug and/or its metabolites. Saliva is thus a suitable biological fluid for determining cocaine, EME, and BEG by GC-PCI-MS.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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