Journal Article

Determination of Clofazimine in Leprosy Patients by High-Performance Liquid Chromatography

R.H.C. Queiroz, R.C. Pereira, M.A. Gotardo, D.S. Cordeiro and E. Melchio

in Journal of Analytical Toxicology

Volume 27, issue 6, pages 377-380
Published in print September 2003 | ISSN: 0146-4760
Published online September 2003 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/27.6.377
Determination of Clofazimine in Leprosy Patients by High-Performance Liquid Chromatography

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An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1, v/v) at pH 3.0, after preciptating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25N sodium acetate buffer at pH 3.0 (74:26, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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