Journal Article

LC-MS Analysis of 2-Oxo-3-Hydroxy LSD from Urine Using a Speedisk<sup>®</sup> Positive-Pressure Processor with Cerex<sup>®</sup> PolyChrom™ CLIN II Columns*

Carl K. Horn, Kevin L. Klette and Peter R. Stout

in Journal of Analytical Toxicology

Volume 27, issue 7, pages 459-463
Published in print October 2003 | ISSN: 0146-4760
Published online October 2003 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/jat/27.7.459
LC-MS Analysis of 2-Oxo-3-Hydroxy LSD from Urine Using a Speedisk® Positive-Pressure Processor with Cerex® PolyChrom™ CLIN II Columns*

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A rapid and sensitive solid-phase extraction method for the lysergic acid diethylamide (LSD) metabolite 2-oxo-3-hydroxy-LSD (O-H-LSD) was developed for use in a forensic laboratory. The method uses a positive.pressure manifold anion-exchange polymer-based solid-phase extraction with subsequent analysis by liquid chromatography-mass spectrometry (LC-MS). The average extraction efficiency was 92%. The limits of detection (LOD) and quantitation (LOQ) were calculated at 250 pg/mL. The assay was linear, precise, and accurate from 250 to 30,000 pg/mL with an r2 of 0.999. Samples including 93 compounds with properties similar to O-H-LSD, common over-the-counter products, prescription drugs and some of their metabolites, and other drugs of abuse were analyzed and produced no significant interference. This method has increased our efficiency in analyzing O-H-LSD by reducing the overall extraction and analysis time. The increase in extraction efficiency enabled decreased assay LOD and LOQ values while lowering the volume required for injection.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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